Australia captain and forward Sam Kerr was ruled out of two games of the Women's World Cup, including the opener against Ireland on Thursday, after picking up a calf injury in training a day earlier.
Kerr's loss is a big blow for Tony Gustavsson's 10th-ranked Matildas, who are considered one of the favourites to win their home tournament. The 29-year-old Chelsea striker is the all time leading goalscorer for her country with 63 goals.
The team said she will be re-assessed after Australia's second group stage match on July 27 against Nigeria.
Australia kicked off the tournament on a wave of momentum having won nine of their previous 10 games, including a 1-0 win over France in a friendly last Friday and a 2-0 win over England in April that ended the Lionesses' 30-game unbeaten streak.
There was some injury speculation around Kerr after the France game when she was spotted with an ice pack on her hamstring after she was taken off in the 62nd minute.
This is the fourth World Cup for Kerr, who spoke about the pride of playing on the global stage in front of a home crowd.
"To be at home and get the feel for it, walk down the streets and see people that are excited or flying around the country to watch us, it's nice to be able to feel the love," she said on Wednesday.
"But also we're really proud to bring this World Cup home and let Australia experience what we do all around the world."